Page 23: of Marine Technology Magazine (March 2020)

select sample analysis and sample The use of micro? uidics for in situ

HQTXLULHV#EOXHSULQWVXEVHDFRP preservation. Unlike its predecessors, nutrient sensing has gained a great

VRXQGE\GHVLJQ however, the new ESP design concept deal of traction in recent years. Mi- is inherently more modular, with sam- cro? uidic systems require less power, ple collection and handling distinct reagent, time, and human involvement from sensors needed for real-time ana- than conventional instruments, mak- lytical work. This new ESP employs ing them ideal for long term deploy- 0XOWLEHDP,PDJLQJ6RQDUV 6LQJOHDQG'XDO)UHTXHQF\WRN+] single-use cartridges containing both ments. These concepts are being em- media for concentrating particles and ployed to eDNA by the Sieben Lab at reagents for processing that material. Dalhousie University in Nova Scotia.

The interface between the cartridges Their “Inlaid Optical Celltm” technol- and the sampler has a ? xed design. ogy is a patent-pending approach that

Cartridges can evolve to meet new enables miniaturized optical spectros- needs so long as they meet the inter- copy on ultra-small amounts of ? uid, face speci? cation. Two types of ESP less than a few microliters. Conserv- cartridges have been devised. One is ing reagent usage per measurement meant for sample preservation only, minimizes the volume of onboard while the other is designed for homog- chemicals and waste storage leading enizing collected material and passage to smaller sensors. These inlaid cells to downstream analytical modules. are used to perform absorbance and

This cartridge based design has been ? uorescence measurements. In these tested at sea on the MBARI LRAUV. systems, on-chip valving and pump- 0LFUR86%/7UDFNLQJ%HDFRQV

Illustration showing how the third-generation Environmental Sample %LGLUHFWLRQDO$FRXVWLF'DWD0RGHPV

Processor mounts inside MBARI’s long-range AUV 3RUWDEOH6LGHVFDQ6RQDUV 7RZHG2(0+XOODQG3ROH0RXQWHG

ILQGRXWPRUH (Credit: MBARI)

ZZZEOXHSULQWVXEVHDFRP www.marinetechnologynews.com

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